We mainly focus on ….
(1) Molecular basis of regeneration (e.g., Spinal cord and caudal fin). Research organism: African killifish N. furzeri.
(2) Evolution of regenerative capacities in vertebrates. Research organisms: Teleost fish, mice, and rabbits
(3) Regeneration and rejuvenation. Research organism: African killifish N. furzeri.
–Genetic Models–
Regeneration has long attracted biomedical interest because of the potential of replacing damaged organs with new ones. However, why some lower vertebrates (e.g., fish and salamanders) regenerate extensively while others such as mammals regenerate poorly is not well understood.
In addition to the popular mouse model, the Wang lab is also powered with a new genetic model, the African killifish Nothobranchius furzeri.
We are deploying comparative studies and genetic screens to decode the failure of regeneration in non-regenerative animals. The Wang Lab is interested in identifying molecular mechanisms that can be targeted to help humans rebuild damaged organs.
The African killifish inhabit temporal ponds subjected to annual desiccation in the southeast of Africa. Adult fish are only present in the rainy season when the ponds are filled with water. The African killifish survive the dry season as diapause embryos buried in the mud, waiting for the next rainy season to hatch and reproduce again. The strong selective pressures have driven this species to evolve unique features that can expedite organ development-, regeneration- and aging-related studies including 1) a remarkably short generation time (30-45 days); 2) diapause embryos that allow for convenient maintenance and storage of strains/lines in dry Petri Dishes for years ; 3) rapid aging under laboratory conditions (a median lifespan of 4-6 months); and 4) highly efficient genetic manipulations with CRISPR/Cas9 and Tol2-mediated transgenesis. The African killifish provides a unique opportunity to explore adult regeneration and aging much faster than what is possible with other model organisms.